Publications

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Interstitial fluid flow in an engineered human lymph node stroma model modulates T cell egress and stromal change Open Access

Jennifer H. Hammel, Abhinav Arneja, Jessica Cunningham, Maosen Wang, Sophia Schumaecker, Yamilet Macias Orihuela, Tochukwu Ozulumba, Jonathan M. Zatorski, Thomas J. Braciale, Chance John Luckey, Rebecca R. Pompano, Jennifer M. Munson; Interstitial fluid flow in an engineered human lymph node stroma model modulates T cell egress and stromal change. APL Bioeng. 1 June 2025; 9 (2): 026105. https://doi.org/10.1063/5.0247363

Transient Lymphatic Remodeling Follows Sub-Ablative High-Frequency Irreversible Electroporation Therapy in a 4T1 Murine Model

Esparza, S., Jacobs, E., Hammel, J.H. et al. Transient Lymphatic Remodeling Follows Sub-Ablative High-Frequency Irreversible Electroporation Therapy in a 4T1 Murine Model. Ann Biomed Eng 53, 1148–1164 (2025). https://doi.org/10.1007/s10439-024-03674-y

Interstitial fluid transport dynamics predict glioblastoma invasion and progression

Cora M. Carman-Esparza, Caleb A. Stine, Naciye Atay, Kathryn M. Kingsmore, Maosen Wang, Ryan T. Woodall, Russell C. Rockne, Jessica J. Cunningham, Jennifer M. Munson bioRxiv 2025.03.12.642840; doi: https://doi.org/10.1101/2025.03.12.642840

Ex Vivo Model of Breast Cancer Cell Invasion in Live Lymph Node Tissue

Katerina Morgaenko, Abhinav Arneja, Alexander G. Ball, Audrey M. Putelo, Jennifer M. Munson, Melanie R. Rutkowski, and Rebecca R. Pompano, ACS Pharmacology & Translational Science 2025 8 (3), 690-705, DOI: 10.1021/acsptsci.4c00431

Initiation of primary T cell—B cell interactions and extrafollicular antibody responses in an organized microphysiological model of the human lymph node

Jonathan M. Zatorski, Djuro Raskovic, Abhinav Arneja, Saweetha Kiridena, Tochukwu Ozulumba, Jennifer H. Hammel, Parastoo Anbaei, Jennifer E. Ortiz-Cárdenas, Thomas J. Braciale, Jennifer M. Munson, Chance John Luckey, Rebecca R. Pompano bioRxiv 2025.01.12.632545; doi: https://doi.org/10.1101/2025.01.12.632545

5 - The immune system and its role in the nervous system

Gabriela Geraldo Mendes, Samantha Howerton, Jennifer Munson, 5 - The immune system and its role in the nervous system, Editor(s): Stephanie Willerth, Handbook of Neural Engineering, Academic Press, 2025, Pages 149-177, ISBN 9780323957304, https://doi.org/10.1016/B978-0-323-95730-4.00014-7.

Engineered human lymph node stroma model for examining interstitial fluid flow and T cell egress

Jennifer H Hammel, Abhinav Arneja, Jessica Cunningham, Maosen Wang, Sophia Schumaecker, Yamilet Macias Orihuela, Tochukwu Ozulumba, Jonathan Zatorski, Thomas J Braciale, Chance John Luckey, Rebecca R Pompano, Jennifer M Munson bioRxiv 2024.12.03.622729; doi: https://doi.org/10.1101/2024.12.03.622729

A novel methodology for mapping interstitial fluid dynamics in murine brain tumors using DCE-MRI

Cora Carman-Esparza, Kathryn Kingsmore, Andrea Vaccari, Skylar Davis, Jessica Cunningham, Maosen Wang, Jennifer Munson, A novel methodology for mapping interstitial fluid dynamics in murine brain tumors using DCE-MRI, Methods, Volume 231, 2024, Pages 78-93, ISSN 1046-2023, https://doi.org/10.1016/j.ymeth.2024.09.008.

A Photopolymerizable Hyaluronic Acid-Collagen Model of the Invasive Glioma Microenvironment with Interstitial Flow

Howerton, S., Liang, Y., Hammel, J., Purow, B., Munson, J. A Photopolymerizable Hyaluronic Acid-Collagen Model of the Invasive Glioma Microenvironment with Interstitial Flow. J. Vis. Exp. (212), e66604, doi:10.3791/66604 (2024).

Model discovery approach enables noninvasive measurement of intra-tumoral fluid transport in dynamic MRI Open Access

Ryan T. Woodall, Cora C. Esparza, Margarita Gutova, Maosen Wang, Jessica J. Cunningham, Alexander B. Brummer, Caleb A. Stine, Christine C. Brown, Jennifer M. Munson, Russell C. Rockne; Model discovery approach enables noninvasive measurement of intra-tumoral fluid transport in dynamic MRI. APL Bioeng. 1 June 2024; 8 (2): 026106. https://doi.org/10.1063/5.0190561

Structural and practical identifiability of contrast transport models for DCE-MRI

Conte M, Woodall RT, Gutova M, Chen BT, Shiroishi MS, Brown CE, et al. (2024) Structural and practical identifiability of contrast transport models for DCE-MRI. PLoS Comput Biol 20(5): e1012106. https://doi.org/10.1371/journal.pcbi.1012106

Abstract 2581: Measuring interstitial fluid flow to predict and optimize spatial CAR-T dynamics in the clinic Available

Ryan T. Woodall, Cora Esparza, Christine C. Brown, Jennifer M. Munson, Russell C. Rockne. Measuring interstitial fluid flow to predict and optimize spatial CAR-T dynamics in the clinic [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2024; Part 1 (Regular Abstracts); 2024 Apr 5-10; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2024;84(6_Suppl):Abstract nr 2581.

Mitigating reactive oxygen species production and increasing gel porosity improves lymphocyte motility and fibroblast spreading in photocrosslinked gelatin-thiol hydrogels

Tochukwu Ozulumba, Jonathan M. Zatorski, Abhinav Arneja, Jennifer H. Hammel, Thomas J. Braciale, Chance J. Luckey, Jennifer M. Munson, Rebecca R. Pompano
bioRxiv 2024.01.14.574282; doi: https://doi.org/10.1101/2024.01.14.574282

Demonstration of chemotherapeutic mediated lymphatic changes in meningeal lymphatics in vitro, ex vivo, and in vivo

L. Monet Roberts, Jennifer H Hammel, Francesca Azar, Tzu-Yu (Alkaid) Feng, Jessica J. Cunningham, Melanie Rutkowski, Jennifer Munson

bioRxiv 2024.01.06.574460; doi: https://doi.org/10.1101/2024.01.06.574460

Pregnancy-induced remodeling of the murine reproductive tract: a longitudinal in vivo magnetic resonance imaging study

Suarez, A.C., Gimenez, C.J., Russell, S.R. et al. Pregnancy-induced remodeling of the murine reproductive tract: a longitudinal in vivo magnetic resonance imaging study. Sci Rep 14, 586 (2024). https://doi.org/10.1038/s41598-023-50437-1

Structural and practical identifiability 1 of contrast transport models for DCE-MRI

Martina Conte, Ryan T. Woodall, Margarita Gutova, Bihong T. Chen, Mark S. Shiroishi, Christine E. Brown, Jennifer M. Munson, and Russell C. Rockne, bioRxiv, Page v282, https://doi.org/10.1101/2023.12.19.572294

TMIC-21. SPHINGOSINE-1-PHOSPHATE RECEPTOR 3 (S1PR3) IN THE GLIOBLASTOMA TUMOR MICROENVIRONMENT MAY PLAY A ROLE IN IMMUNOSUPPRESSION

Sharon Michelhaugh, Jennifer Munson, TMIC-21. SPHINGOSINE-1-PHOSPHATE RECEPTOR 3 (S1PR3) IN THE GLIOBLASTOMA TUMOR MICROENVIRONMENT MAY PLAY A ROLE IN IMMUNOSUPPRESSION, Neuro-Oncology, Volume 25, Issue Supplement_5, November 2023, Page v282, https://doi.org/10.1093/neuonc/noad179.1087

TMIC-64. CHARACTERIZATION OF THE TUMOR MICROENVIRONMENT IN DIFFUSE MIDLINE GLIOMAS (DMG) FOR 3D IN VITRO MODEL BUILDING

Sarah Kremer, Rhea John, Peng Jin, Sharon Michelhaugh, Jessica Cunningham, Samantha Howerton, Augustine Eze, Javad Nazarian, Jennifer Munson, TMIC-64. CHARACTERIZATION OF THE TUMOR MICROENVIRONMENT IN DIFFUSE MIDLINE GLIOMAS (DMG) FOR 3D IN VITRO MODEL BUILDING, Neuro-Oncology, Volume 25, Issue Supplement_5, November 2023, Pages v292–v293, https://doi.org/10.1093/neuonc/noad179.1130

580 Mapping interstitial fluid flow in the brain to improve CAR T cell trafficking and efficacy

Margarita Gutova, Ryan Woodall, Eric Ma, Cora Esparza, Vanessa Salvary, Brenda Aguilar, Renate Starr, Behnam Badie, Darya Alizadeh, Jennifer M Munson, Russell C Rockne, Christine Brown - 580 Mapping interstitial fluid flow in the brain to improve CAR T cell trafficking and efficacy: Journal for ImmunoTherapy of Cancer 2023;11:.

Model discovery approach enables non-invasive measurement of intra-tumoral fluid transport in dynamic MRI

Ryan T. Woodall, Cora C. Esparza, Margarita Gutova, Maosen Wang, Jessica J. Cunningham, Alexander B. Brummer, Caleb A. Stine, Christine C. Brown, Jennifer M. Munson, Russell C. Rockne

bioRxiv 2023.08.28.554919; doi: https://doi.org/10.1101/2023.08.28.554919

Development of a Synthetic, Injectable Hydrogel to Capture Residual Glioblastoma and Glioblastoma Stem-Like Cells with CXCL12-Mediated Chemotaxis

Z. M. Khan, J. M. Munson, T. E. Long, E. Vlaisavljevich, S. S. Verbridge, Development of a Synthetic, Injectable Hydrogel to Capture Residual Glioblastoma and Glioblastoma Stem-Like Cells with CXCL12-Mediated Chemotaxis. Adv. Healthcare Mater. 2023, 12, 2300671. https://doi.org/10.1002/adhm.202300671

Modeling lymphangiogenesis: Pairing in vitro and in vivo metrics

Suarez AC, Hammel JH, Munson JM. Modeling lymphangiogenesis: Pairing in vitro and in vivo metrics. Microcirculation. 2023; 30:e12802. doi:10.1111/micc.12802

Non-invasive measurement of intra-tumoral fluid dynamics with localized convolutional function regression

Ryan T. Woodall, Cora C. Esparza, Margarita Gutova, Maosen Wang, Jessica J. Cunningham, Alexander B. Brummer, Caleb A. Stine, Christine C. Brown, Jennifer M. Munson, Russell C. Rockne

bioRxiv 2023.08.28.554919; doi: https://doi.org/10.1101/2023.08.28.554919

Fluids and flows in brain cancer and neurological disorders

Jin, P., & Munson, J. M. (2023). Fluids and flows in brain cancer and neurological disorders. WIREs Mechanisms of Disease, 15(1), e1582. https://doi.org/10.1002/wsbm.1582

Towards spatially-organized organs-on-chip: Photopatterning cell-laden thiol-ene and methacryloyl hydrogels in a microfluidic device

Jennifer E. Ortiz-Cárdenas, Jonathan M. Zatorski, Abhinav Arneja, Alyssa N. Montalbine, Jennifer M. Munson, Chance John Luckey, Rebecca R. Pompano, Towards spatially-organized organs-on-chip: Photopatterning cell-laden thiol-ene and methacryloyl hydrogels in a microfluidic device, Organs-on-a-Chip, Volume 4, 2022, 100018, ISSN 2666-1020, https://doi.org/10.1016/j.ooc.2022.100018.

Fusobacterium nucleatum induces proliferation and migration in pancreatic cancer cells through host autocrine and paracrine signaling

Barath Udayasuryan, Raffae N Ahmad, Tam TD Nguyen, Ariana Umaña, LaDeidra Monét Roberts, Polina Sobol, Stephen D Jones, Jennifer M Munson, Daniel J Slade, Scott S Verbridge Fusobacterium nucleatum induces proliferation and migration in pancreatic cancer cells through host autocrine and paracrine signaling.Sci. Signal.15,eabn4948(2022).DOI:10.1126/scisignal.abn4948

Patient-designed models of the invasive edge of glioblastoma

RC Cornelison, JX Yuan, KM Tate, A Petrosky†, GF Beeghly, M Bloomfield, SC Schwager†, AL Berr†, D Cimini, FF Bafakih, JW Mandell, BW Purow, BJ Horton, JM Munson*. A patient-designed tissue-engineered model of the infiltrative glioblastoma microenvironment, NPJ Precision Oncology.6(54) 2022. Available: https://rdcu.be/cXtxU

Cell migration in response to flow and endothelial cell interactions

L. M. Roberts, M. J. Perez, K. N. Balogh, G. Mingledorff, J. V. Cross, and J. M. Munson, “Myeloid Derived Suppressor Cells Migrate in Response to Flow and Lymphatic Endothelial Cell Interaction in the Breast Tumor Microenvironment,” Cancers, vol. 14, no. 12, Art. no. 12, Jan. 2022, doi: 10.3390/cancers14123008.

Lymphangiogenesis to be prevented with Anti-VEGFR3 therapy

A. R. Harris et al., “Platinum Chemotherapy Induces Lymphangiogenesis in Cancerous and Healthy Tissues That Can be Prevented With Adjuvant Anti-VEGFR3 Therapy,” Frontiers in Oncology, vol. 12, 2022, Accessed: Jun. 27, 2022. [Online]. Available: https://www.frontiersin.org/article/10.3389/fonc.2022.801764

Repeatability of tumor perfusion kinetics

R. T. Woodall et al., “Repeatability of tumor perfusion kinetics from dynamic contrast-enhanced MRI in glioblastoma,” Neuro-Oncology Advances, vol. 3, no. 1, p. vdab174, Jan. 2021, doi: 10.1093/noajnl/vdab174.

Gradient formation in interstitial fluid flow environments

C. A. Stine and J. M. Munson, “Autologous Gradient Formation under Differential Interstitial Fluid Flow Environments,” Biophysica, vol. 2, no. 1, Art. no. 1, Mar. 2022, doi: 10.3390/biophysica2010003.

Tissue engineering models of breast cancer

J. A. McGuire et al., “Tear propagation in vaginal tissue under inflation,” Acta Biomaterialia, vol. 127, pp. 193–G. F. Beeghly, C. Thomas, J. X. Yuan, A. R. Harris, and J. M. Munson, “Designing Patient-Driven, Tissue-Engineered Models of Primary and Metastatic Breast Cancer,” Bioengineering, vol. 9, no. 2, Art. no. 2, Feb. 2022, doi: 10.3390/bioengineering9020044.

Engineering advances for women’s health

R. De Vita and J. Munson, “Special Issue on the Advances in Engineering for Women’s Health,” Ann Biomed Eng, vol. 49, no. 8, pp. 1785–1787, Aug. 2021, doi: 10.1007/s10439-021-02837-5.

Tear propagation in vaginal tissue

J. A. McGuire et al., “Tear propagation in vaginal tissue under inflation,” Acta Biomaterialia, vol. 127, pp. 193–204, Jun. 2021, doi: 10.1016/j.actbio.2021.03.065.

Ultrasound promotes nanoparticle dispersion and transfection

Curley T., Colleen et al. Augmentation of brain tumor interstitial flow via focused ultrasound promotes brain-penetrating nanoparticle dispersion and transfection. Science Advances, vol. 6, no. 18, p. eaay1344, May 2020, doi: 10.1126/sciadv.aay1344.

Measuring interstitial fluid flow in glioblastoma patients

Atay, Chatterjee, et al. Utilizing Dynamic Contrast-Enhanced Magnetic Resonance Imaging (DCE-MRI) to Analyze Interstitial Fluid Flow and Transport in Glioblastoma and the Surrounding Parenchyma in Human Patients. Pharmaceutics. 13 (2): 212 (2021). doi: 10.3390/pharmaceutics13020212

Focused Ultrasound-mediated blood brain barrier opening increases interstitial fluid flow

Curley, et al. Augmentation of brain tumor interstitial flow via focused ultrasound promotes brain-penetrating nanoparticle dispersion and transfection. Science Advances.6(18): eaay1344 (2020). DOI: 10.1126/sciadv.aay1344

Review: Engineering the tumor microenvironment

LM Roberts, JM Munson, Modulating microenvironments for treating glioblastoma. Current tissue microenvironment reports. 1, 99-111 (2020)

Review: Studying fluid flow in the brain

KC Chatterjee, CA Carman-Esparza, JM Munson, Methods to measure, model, and manipulate fluid flow in the brain. Neuroscience Methods. 333: 108541. (2020) https://doi.org/10.1016/j.jneumeth.2019.108541

Review: Linking CED to interstitial flow

CA Stine, JM Munson, Convection enhanced delivery: role of increased interstitial fluid flow. Frontiers: Oncology. 9: 966 (2019).

Opinion: Can interstitial fluid flow be a drug target?

JM Munson, Interstitial fluid flow under the microscope: is it a future drug target for high grade brain tumours such as glioblastoma? Expert Opinion on Therapeutic Targets, 23 (9), 725-728(2019). DOI: 10.1080/14728222.2019.1647167

Recommendations for assessing drug response in 3D

EA Brooks, S Galarza, MF Gencoglu, RC Cornelison, JM Munson, SR Peyton, Applicability of drug response metrics for cancer studies using biomaterials. Phil Transactions of the Royal Society B. 374(1779), 20180226 (2019). DOI:10.1101/408583.

Review: Systems for testing drugs for nervous disorders

1. KM Tate, JM Munson, Assessing drug response in engineered neural microenvironments. Brain Research Bulletin. 150: 21-34 (2019). DOI: 10.1016/j.brainresbull.2019.04.027

A novel lymph node-tumor on a chip system

1. S Shim, M Balanger, AR Harris, JM Munson, RR Pompano, Two-way communication between ex vivo tissues on a microfluidic chip: application to tumor-lymph node interaction. Lab on a chip. 19, 1013-1026 (2019). DOI: 10.1039/C8LC00957K .

Increased fluid flow in vivo leads to increased glioma invasion

RC Cornelison, KM Kingsmore, CR Brennan, JM Munson, Functional aspects of meningeal lymphatics in ageing and Alzheimer’s disease. Nature. (2018). DOI: 10.1038/s41586-018-0368-8.

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Increasing meningeal lymphangiogenesis to boost memory

S Da Mesquita, A Louveau, A Vaccari, I Smirnov, RC Cornelison, KM Kingsmore, C Contarino, S Onengut-Gumuscu, E Farber, D Raper, KE Viar, W Baker, N Dabhi, G Oliver, S Rich, JM Munson, CC Overall, ST Acton, J Kipnis, Functional aspects of meningeal lymphatics in ageing and Alzheimer’s disease. Nature. (2018). DOI: 10.1038/s41586-018-0368-8.